View of apparent genetic DNA in skin cells

View of apparent genetic DNA in skin cells

ScienceDaily (18 November, 2012) - Current wisdom is that every cell in the body contains the same DNA. However, stem cells derived from skin, a new study found that genetic variation in body tissues, with deep implications for genetic screening in search of a large-scale researchers at Yale School of Medicine The're's.

A study published in the November 18 issue of Nature, gene conversion, and better understanding of human development and a way to evaluate the extent of disease paves.

"We found that human cells with different genomes are made of a masterpiece," lead author Dr. Flora Vaccarino, professor of child psychiatry at the Yale Child Study Center, Harris said.

Long-standing belief that our cells have the same DNA sequence and blueprint controls body functions. Yale research team believe this challenge. Some scientists hypothesized that during development, when cells from mother to daughter DNA is copied, deleted, duplications and may change in DNA sequence, and gene can affect an entire group. The base is incredibly difficult to evaluate, but this new research Vaccarino and her colleagues have also done so.

Induced pluripotent stem cell lines, the entire team (IPS), which developed from cells of different adult stem cells are used to study genome sequence. The two families grew cells taken from the inner upper arms. The team spent two years IPS cell lines characterizing these cells and compare them to the original as possible.

While the genome of the IPS cells from skin cells of origin of the genome is close, the team several deletions or duplications of DNA thousands of base pairs can be identified to include. The team performed additional experiments to understand the differences, and shows that at least half of them are already in the skin cells smaller products. These differences IPS IPS cells revealed because one line, or very little, is derived from skin cells has been. Vaccarino said his lines IPS acts as a magnifying glass genomic differences in body cells can watch the scene.

He has extensive skin and skin cell mosaicism in at least 30 per cent different DNA deletion or duplication, a small percentage of cells found in every port, "Vaccarino said." Somatic mosaicism of the observed genetic analysis, which currently only using blood samples for blood far-reaching consequences when we look at the DNA, the DNA of other tissues such as brain, not exactly reflect . Mutations Maybe we were missing. '

Vaccarino as "Read" Mark Gerstein, Sherman Weissman, Alexander Eckehart people including civilians worked with a team of researchers from laboratories, Neurodevelopment and regeneration program to work under supervision. Other authors on the study Alexej Abyzov, Jessica Mariani, Dean Palejev, Young Jong, Michael Seamus Haney, Livia Tomasini, Anthony Ferrandino, Lior A. Rosenberg Belmaker, Anna Szekely, Michael Wilson, Arif Kocabas, Nathaniel E. Calixto, including Elena L Grigorenko, Anita Huttner, and Katarzyna Chawarska.

Study NIH / NIMH, Simons Foundation, and is funded by the Connecticut State.